| Date: | 4–10 July |
| Edition: | 9th |
| Surface: | Clay |
| Location: | Cali, Colombia |
| Champs: | Darian King |
| Champd: | Nicolás Jarry / Hans Podlipnik |
| Before Name: | Seguros Bolívar Open Cali |
The 2016 Milo Open Cali was a professional tennis tournament played on clay courts. It was the ninth edition of the tournament, which is part of the 2016 ATP Challenger Tour. It took place in Cali, Colombia between 4 and 10 July 2016.
| Country | Player | Rank | Seed | |
|---|---|---|---|---|
 Dominican Republic | Víctor Estrella Burgos | 77 | 1 | |
 Colombia | Alejandro González | 154 | 2 | |
| Gonzalo Lama | 161 | 3 | ||
| Colombia | Eduardo Struvay | 162 | 4 | |
 Brazil | João Souza | 183 | 5 | |
| Marcelo Arévalo | 218 | 6 | ||
| Darian King | 255 | 7 | ||
 Argentina | Juan Ignacio Londero | 258 | 8 |
The following players received wildcards into
Giovanni Lapentti Felipe Mantilla Nicolás Mejía Cristian RodríguezThe following player entered the singles main draw with a protected ranking:
The following players received entry from the qualifying draw:
| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
| Brazil | João Souza | 289 | 1 | |||
| Colombia | Nicolás Barrientos |  Venezuela, Bolivarian Republic of | Roberto Maytín | 345 | 2 | |
| Nicolás Jarry | Hans Podlipnik | 542 | 3 | |||
| Christopher Díaz Figueroa | Venezuela, Bolivarian Republic of | Luis David Martínez | 581 | 4 |
The following pairs received wildcards into the singles main draw:
José Daniel Bendeck / Nicolás Mejía Alejandro Gómez / Felipe Mantilla Juan Sebastián Gómez / Cristian RodríguezSee main article: 2016 Milo Open Cali – Singles.
Víctor Estrella Burgos, 5–7, 6–4, 7–5See main article: 2016 Milo Open Cali – Doubles.
Erik Crepaldi / Daniel Dutra da Silva, 6–1, 7–6(8–6)