The 2016 Milex Open was a professional tennis tournament played on green clay courts. It was the second edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Santo Domingo, Dominican Republic between 8 February and 14 February 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Dominican Republic | Víctor Estrella Burgos | 58 | 1 | |
 Argentina | Horacio Zeballos | 118 | 2 | |
 Austria | Gerald Melzer | 120 | 3 | |
 Spain | Roberto Carballés Baena | 129 | 4 | |
 Slovakia | Andrej Martin | 141 | 5 | |
 Colombia | Alejandro González | 151 | 6 | |
 Brazil | André Ghem | 153 | 7 | |
| Brazil | João Souza | 154 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry into the singles main draw as alternates:
The following players received entry from the qualifying draw:
See main article: 2016 Milex Open – Singles.
Guido Andreozzi def. Nicolás Kicker, 6–0, 6–4See main article: 2016 Milex Open – Doubles.
Giovanni Lapentti def. 
Jonathan Eysseric / Franko Škugor, 7–5, 6–4