The 2016 Marburg Open was a professional tennis tournament played on clay courts. It was the seventh edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Marburg, Germany between 27 June and 4 July 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Argentina | 1 | |||
 Georgia | Nikoloz Basilashvili | 105 | 2 | |
 Austria | Gerald Melzer | 107 | 3 | |
 Belgium | Steve Darcis | 110 | 4 | |
 Slovakia | Jozef Kovalík | 128 | 5 | |
| Daniel Brands | 130 | 6 | ||
| Tobias Kamke | 133 | 7 | ||
 Spain | Daniel Muñoz de la Nava | 134 | 8 | |
| Argentina | Nicolás Kicker | 135 | 9 |
The following players received wildcards into the singles main draw:
Manuel Peña LópezThe following player received entry as an alternate:
The following players received entry from the qualifying draw:
The following player received entry as a lucky loser:
| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
| James Cerretani | Austria | Philipp Oswald | 180 | 1 | ||
 Poland | Tomasz Bednarek |  Belarus | Sergey Betov | 236 | 2 | |
 Brazil | Fabrício Neis | Ruan Roelofse | 247 | 3 | ||
 Mexico | Miguel Ángel Reyes-Varela | Max Schnur | 263 | 4 |
The following pairs received wildcards into the doubles main draw:
The following pair received entry courtesy of a protected ranking into the doubles main draw:
See main article: 2016 Marburg Open – Singles.
Jan Šátral def. Marco Trungelliti, 6–2, 6–4See main article: 2016 Marburg Open – Doubles.
James Cerretani / Philipp Oswald def. Miguel Ángel Reyes-Varela / Max Schnur, 6–3, 6–2
