| Date: | 11–17 April |
| Edition: | 4th |
| Category: | ITF Women's Circuit |
| Location: | Istanbul, Turkey |
| Prize Money: | $50,000 |
| Champs: |  Barbora Štefková |
| Champd: |  Nigina Abduraimova / Barbora Štefková |
The 2016 Lale Cup was a professional tennis tournament played on outdoor hard courts. It was the fourth edition of the tournament and part of the 2016 ITF Women's Circuit, offering a total of $50,000 in prize money. It took place in Istanbul, Turkey, on 11–17 April 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Hsieh Su-wei | 81 | 1 | ||
 Serbia | Ivana Jorović | 151 | 2 | |
 Russian Federation | Polina Leykina | 189 | 3 | |
 China | Lu Jiajing | 198 | 4 | |
| Russian Federation | Anastasiya Komardina | 216 | 5 | |
| Uzbekistan | Nigina Abduraimova | 217 | 6 | |
 Romania | Cristina Dinu | 222 | 7 | |
 Hungary | Réka-Luca Jani | 225 | 8 |
The following players received wildcards into the singles main draw:
Başak Akbaş İnci Öğüt Selin Övünç Pemra ÖzgenThe following players received entry from the qualifying draw:
See main article: 2016 Lale Cup – Singles.
Barbora Štefková def. Anastasia Pivovarova, 7–5, 2–6, 6–1See main article: 2016 Lale Cup – Doubles.
Nigina Abduraimova / Barbora Štefková def. Valentyna Ivakhnenko / 
Lidziya Marozava, 6–4, 1–6, [10–6]
