| Date: | 25–31 July |
| Edition: | 21st (men) 19th (women) |
| Category: | ATP Challenger Tour ITF Women's Circuit |
| Location: | Lexington, Kentucky, United States |
| Prize Money: | $50,000 (men) $50,000 (women) |
| Champms: |  Ernesto Escobedo |
| Champws: | Michaëlla Krajicek |
| Champmd: |  Luke Saville / Jordan Thompson |
| Champwd: |  Hiroko Kuwata /  Zhu Lin |
The 2016 Kentucky Bank Tennis Championships was a professional tennis tournament played on outdoor hard courts. It was the 21st edition, for men, and 19th edition, for women, of the tournament and part of the 2016 ATP Challenger Tour and the 2016 ITF Women's Circuit, offering totals of $50,000, for both genders, in prize money. It took place in Lexington, Kentucky, United States, on 25–31 July 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 France | Adrian Mannarino | 55 | 1 | |
 Tunisia | Malek Jaziri | 63 | 2 | |
 United Kingdom | Daniel Evans | 82 | 3 | |
| Australia | Jordan Thompson | 90 | 4 | |
| United States | Bjorn Fratangelo | 108 | 5 | |
| France | Quentin Halys | 140 | 6 | |
| United States | Frances Tiafoe | 156 | 7 | |
| United States | Stefan Kozlov | 163 | 8 |
The following players received a wildcard into the singles main draw:
The following player entered as an alternate:
The following players received entry from the qualifying draw:
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Belgium | Alison Van Uytvanck | 116 | 1 | |
 Czech Republic | 2 | |||
 Romania | 3 | |||
| United States | Taylor Townsend | 148 | 4 | |
| Japan | Hiroko Kuwata | 154 | 5 | |
| China | Zhu Lin | 151 | 6 | |
| United States | Grace Min | 158 | 7 | |
| Japan | Miyu Kato | 182 | 8 | |
| Belgium | An-Sophie Mestach | 189 | 9 |
The following players received a wildcard into the singles main draw:
Francesca Di Lorenzo Raveena Kingsley Jamie Loeb Melanie OudinThe following players received entry from the qualifying draw:
The following players received entry as lucky losers:
See main article: article and 2016 Kentucky Bank Tennis Championships – Men's singles.
Ernesto Escobedo def. Frances Tiafoe, 6–2, 6–7(6–8), 7–6(7–3)See main article: article and 2016 Kentucky Bank Tennis Championships – Women's singles.
Arina Rodionova, 6–0, 2–6, 6–2See main article: article and 2016 Kentucky Bank Tennis Championships – Men's doubles.
Luke Saville / Jordan Thompson def. Nicolaas Scholtz / Tucker Vorster, 6–2, 7–5See main article: article and 2016 Kentucky Bank Tennis Championships – Women's doubles.
Hiroko Kuwata / Zhu Lin def. Sophie Chang / Alexandra Mueller, 6–0, 7–5
