| Date: | 2–8 May |
| Edition: | 21st |
| Category: | ITF Women's Circuit |
| Location: | Gifu, Japan |
| Prize Money: | $75,000+H |
| Champs: |  Hiroko Kuwata |
| Champd: | Eri Hozumi / Miyu Kato |
The 2016 Kangaroo Cup was a professional tennis tournament played on outdoor hard courts. It was the twenty-first edition of the tournament and part of the 2016 ITF Women's Circuit, offering a total of $75,000+H in prize money. It took place in Gifu, Japan, on 2–8 May 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 China | Wang Qiang | 79 | 1 | |
| China | Duan Yingying | 126 | 2 | |
 Thailand | Luksika Kumkhum | 140 | 3 | |
| Japan | Miyu Kato | 145 | 4 | |
 Belgium | An-Sophie Mestach | 167 | 5 | |
| Japan | Mayo Hibi | 168 | 6 | |
 Korea, Republic of | Jang Su-jeong | 172 | 7 | |
| China | Zhu Lin | 177 | 8 |
The following players received wildcards into the singles main draw:
Mahiro Date Chihiro Muramatsu Miki Ukai Aiko YoshitomiThe following players received entry from the qualifying draw:
Tori Kinard Chihiro Nunome Akiko OmaeThe following player received entry by a protected ranking:
The following player received entry by a junior exempt:
See main article: 2016 Kangaroo Cup – Singles.
Hiroko Kuwata def. Wang Qiang, 6–2, 2–6, 6–4See main article: 2016 Kangaroo Cup – Doubles.
Eri Hozumi / Miyu Kato def. Hiroko Kuwata / Ayaka Okuno, 6–1, 6–2