The 2016 Japan Women's Open (also known as the 2016 Hashimoto Sogyo Japan Women's Open for sponsorship reasons) was a women's tennis tournament played on outdoor hard courts. It was the eighth edition of the Japan Women's Open, and part of the WTA International tournaments of the 2016 WTA Tour. It was held at the Ariake Coliseum in Tokyo, Japan, from September 12 through September 18, 2016.
| Event | W | F | SF | QF | Round of 16 | Round of 32 | width=85 | Q | width=85 | Q3 | width=85 | Q2 | width=85 | Q1 |
| Singles | 280 | 180 | 110 | 60 | 30 | 1 | 18 | 14 | 10 | 1 | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Doubles | 1 |
| width=130 bgcolor=dfe2e9 | Event | width=80 bgcolor=lime | W | width=85 bgcolor=thistle | F | width=85 bgcolor=ffff00 | SF | width=85 bgcolor=ffebcd | QF | width=85 bgcolor=afeeee | Round of 16 | width=85 bgcolor=afeeee | Round of 321 | width=85 | Q | width=85 | Q2 | width=85 | Q1 |
| Singles | $43,000 | $21,400 | $11,300 | $5,900 | $3,310 | $2,925 | $1,005 | $730 | $530 | ||||||||||
| Doubles2 | $12,300 | $6,400 | $3,435 | $1,820 | $960 |
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Japan | Misaki Doi | 32 | 1 | |
 Belgium | Yanina Wickmayer | 38 | 2 | |
 Kazakhstan | Yulia Putintseva | 42 | 3 | |
 Sweden | Johanna Larsson | 47 | 4 | |
| Madison Brengle | 50 | 5 | ||
 China | Zhang Shuai | 51 | 6 | |
| Christina McHale | 55 | 7 | ||
 Ukraine | Kateryna Bondarenko | 59 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
The following players received entry as a lucky loser:
Misa Eguchi → replaced by Antonia Lottner
Irina Falconi → replaced by Kurumi Nara Kirsten Flipkens → replaced by 
Jana Čepelová Nicole Gibbs → replaced by 
Magda Linette
Anett Kontaveit Kristína Kučová → replaced by Naomi Osaka
Tamira Paszek Nao Hibino Wang Qiang → replaced by Maria Sakkari| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
| China | Xu Yifan | China | Zheng Saisai | 46 | 1 | |
| Ukraine | Kateryna Bondarenko | Chuang Chia-jung | 98 | 2 | ||
 Serbia | Aleksandra Krunić |  Czech Republic | Kateřina Siniaková | 99 | 3 | |
| China | Liang Chen | China | Yang Zhaoxuan | 114 | 4 |
The following pairs received wildcards into the doubles main draw:
See main article: 2016 Japan Women's Open – Singles.
Christina McHale def. Kateřina Siniaková, 3–6, 6–4, 6–4[1]See main article: 2016 Japan Women's Open – Doubles.
Shuko Aoyama / Makoto Ninomiya def. 
Jocelyn Rae / Anna Smith, 6–3, 6–3