| Year: | 2016 |
| Team: | James Madison Dukes |
| Sport: | football |
| Conference: | Colonial Athletic Association |
| Short Conf: | CAA |
| Statsrank: | 1 |
| Fcscoachrank: | 1 |
| Record: | 14–1 |
| Conf Record: | 8–0 |
| Head Coach: | Mike Houston |
| Hc Year: | 1st |
| Off Coach: | Donnie Kirkpatrick |
| Oc Year: | 1st |
| Def Coach: | Bob Trott |
| Dc Year: | 1st |
| Stadium: | Bridgeforth Stadium |
| Champion: | NCAA Division I champion CAA champion |
| Bowl: | NCAA Division I Championship Game |
| Bowl Result: | W 28–14 vs. Youngstown State |
The 2016 James Madison Dukes football team represented James Madison University during the 2016 NCAA Division I FCS football season. They were led by first year head coach Mike Houston and played their home games at Bridgeforth Stadium and Zane Showker Field. They were a member of the Colonial Athletic Association (CAA). They finished the season 14–1 overall with an 8–0 mark in CAA play to win the conference title. They received the automatic bid to the FCS playoffs, where they defeated New Hampshire, Sam Houston State, and five-time defending champions North Dakota State to advance to the National Championship Game, where they defeated Youngstown State. This was their first national championship since 2004.
See also: 2016 Morehead State Eagles football team.
See also: 2016 Central Connecticut Blue Devils football team.
See also: 2016 North Carolina Tar Heels football team.
See also: 2016 Maine Black Bears football team.
See also: 2016 Delaware Fightin' Blue Hens football team and Delaware–James Madison football rivalry.
See also: 2016 New Hampshire Wildcats football team.
See also: 2016 Rhode Island Rams football team.
See also: 2016 Richmond Spiders football team.
See also: 2016 Villanova Wildcats football team.
See also: 2016 Elon Phoenix football team.
See also: 2016 Sam Houston State Bearkats football team.
See also: 2016 North Dakota State Bison football team.
It was this game that ended NDSU's streak of five consecutive FCS titles.
See also: 2016 Youngstown State Penguins football team.