| Date: | 14–20 March |
| Edition: | 6th |
| Draw: | 32S / 16D |
| Surface: | Hard |
| Prize Money: | $100,000+H |
| Location: | Guadalajara, Mexico |
| Champs: |  Malek Jaziri |
| Champd: | Gero Kretschmer / Alexander Satschko |
The 2016 Jalisco Open was a professional tennis tournament played on hard courts. It was the sixth edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Guadalajara, Mexico between 14 and 20 March 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Dominican Republic | Víctor Estrella Burgos | 67 | 1 | |
 France | Lucas Pouille | 87 | 2 | |
 Bosnia and Herzegovina | Damir Džumhur | 93 | 3 | |
 Spain | Roberto Carballés Baena | 107 | 4 | |
| Tunisia | Malek Jaziri | 108 | 5 | |
 Argentina | Horacio Zeballos | 113 | 6 | |
 Colombia | Alejandro Falla | 129 | 7 | |
| Daniel Brands | 130 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry into the singles main draw as special exempt:
The following players entered the singles main draw as alternates:
The following players received entry from the qualifying draw:
See main article: 2016 Jalisco Open – Singles.
Malek Jaziri def. Stéphane Robert, 5–7, 6–3, 7–6(7–5)See main article: 2016 Jalisco Open – Doubles.
Santiago González / Mate Pavić, 6–3, 4–6, [10–2]
