| Year: | 2016 |
| Team: | Jacksonville State Gamecocks |
| Sport: | football |
| Conference: | Ohio Valley Conference |
| Short Conf: | OVC |
| Statsrank: | 6 |
| Fcscoachrank: | 6 |
| Record: | 10–2 |
| Conf Record: | 7–0 |
| Head Coach: | John Grass |
| Hc Year: | 3rd |
| Codef Coach1: | David Blackwell |
| Codc1 Year: | 3rd |
| Codef Coach2: | Brandon Hall |
| Codc2 Year: | 3rd |
| Stadium: | Burgess–Snow Field at JSU Stadium |
| Champion: | OVC champion |
| Bowl: | NCAA Division I Second Round |
| Bowl Result: | L 24–40 vs. Youngstown State |
The 2016 Jacksonville State Gamecocks football team represented Jacksonville State University as a member of the Ohio Valley Conference (OVC) during the 2016 NCAA Division I FCS football season. Led by third-year head coach John Grass, the Gamecocks compiled an overall record of 10–2 with a mark of 7–0 in conference play, winning the OVC title for the third consecutive season. Jacksonville State received the OVC's automatic bid to the NCAA Division I Football Championship playoffs. After a first-round bye, the Gamecocks lost in the second round to the eventual national runner-up, Youngstown State. The team played home games at Burgess–Snow Field at JSU Stadium in Jacksonville, Alabama. The team played home games at Burgess–Snow Field at JSU Stadium in Jacksonville, Alabama.
See also: North Alabama Lions football.
See also: 2016 LSU Tigers football team.
See also: 2016 Coastal Carolina Chanticleers football team.
See also: 2016 Liberty Flames football team.
See also: 2016 Tennessee Tech Golden Eagles football team.
See also: 2016 Austin Peay Governors football team.
See also: 2016 Eastern Kentucky Colonels football team.
See also: 2016 Eastern Illinois Panthers football team.
See also: 2016 Southeast Missouri State Redhawks football team.
See also: 2016 Murray State Racers football team.
See also: 2016 UT Martin Skyhawks football team.
See also: 2016 Youngstown State Penguins football team.