The 2016 Israel Open was a professional tennis tournament played on hard courts. It was the 5th edition of the tournament, which was part of the 2016 ATP Challenger Tour. It took place in Ra'anana, Israel between 28 March and 3 April.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Russian Federation | Evgeny Donskoy | 82 | 1 | |
 Israel | Dudi Sela | 84 | 2 | |
 Lithuania | 86 | 3 | ||
 Slovakia | Lukáš Lacko | 98 | 4 | |
 Italy | Thomas Fabbiano | 110 | 5 | |
| Russian Federation | 131 | 6 | ||
 Turkey | Marsel İlhan | 137 | 7 | |
| Marco Chiudinelli | 139 | 8 |
The following players received wildcards into the singles main draw:
Dekel Bar Daniel Cukierman Tal Goldengoren Edan LeshemThe following players received entry as alternates:
The following players received entry from the qualifying draw:
See main article: 2016 Israel Open – Singles.
Evgeny Donskoy def. Ričardas Berankis, 6–4, 6–4See main article: 2016 Israel Open – Doubles.
Konstantin Kravchuk / 
Denys Molchanov def. Jonathan Erlich / 
Philipp Oswald, 4–6, 7–6(7–1), [10–4]
