The 2016 Internazionali di Tennis Città di Vicenza was a professional tennis tournament played on clay courts. It was the third edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Vicenza, Italy between 21 and 29 May May 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Gastão Elias | 98 | 1 | ||
| Daniel Brands | 138 | 2 | ||
 Brazil | Thiago Monteiro | 139 | 3 | |
 Colombia | Alejandro González | 142 | 4 | |
 Argentina | Renzo Olivo | 145 | 5 | |
 Australia | Nicolás Kicker | 150 | 6 | |
 Italy | Luca Vanni | 159 | 7 | |
 Spain | Daniel Gimeno Traver | 161 | 8 |
The following players received wildcards into the singles main draw:
Francisco Bahamonde Lorenzo Sonego Matteo Donati Edoardo EreminThe following players received entry into the singles main draw as special exempt:
The following players entered the singles main draw with a protected ranking:
The following player entered as an alternate:
The following players received entry from the qualifying draw:
See main article: 2016 Internazionali di Tennis Città di Vicenza – Singles.
Guido Andreozzi def. Pere Riba, 6–0, ret.See main article: 2016 Internazionali di Tennis Città di Vicenza – Doubles.
Andrey Golubev / Nikola Mektić def. Gastão Elias / Fabrício Neis, 6–3, 6–3
