| Date: | 30 May–5 June |
| Edition: | 9th |
| Category: | ITF Women's Circuit |
| Location: | Brescia, Italy |
| Prize Money: | $50,000 |
| Champs: |  Karin Knapp |
| Champd: | Deborah Chiesa / Martina Colmegna |
The 2016 Internazionali Femminili di Brescia was a professional tennis tournament played on outdoor clay courts. It was the ninth edition of the tournament and part of the 2016 ITF Women's Circuit, offering a total of $50,000 in prize money. It took place in Brescia, Italy, on 30 May–5 June 2016.[1]
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Italy | Karin Knapp | 118 | 1 | |
 Japan | Risa Ozaki | 128 | 2 | |
| Romina Oprandi | 137 | 3 | ||
 Ukraine | Maryna Zanevska | 141 | 4 | |
 Austria | Barbara Haas | 168 | 5 | |
| Cindy Burger | 173 | 6 | ||
 Spain | Sara Sorribes Tormo | 191 | 7 | |
 United States | Kristie Ahn | 193 | 8 |
The following player received a wildcard into the singles main draw:
The following players received entry from the qualifying draw:
See main article: 2016 Internazionali Femminili di Brescia – Singles.
Karin Knapp def. 
Jesika Malečková, 6–1, 6–2See main article: 2016 Internazionali Femminili di Brescia – Doubles.
Deborah Chiesa / Martina Colmegna def. Cindy Burger / 
Stephanie Vogt, 6–3, 1–6, [12–10]
