| Champ: |  Andy Murray |
| Runner: |  Novak Djokovic |
| Score: | 6–3, 6–3 |
See main article: 2016 Italian Open (tennis).
Andy Murray defeated the two-time defending champion Novak Djokovic in the final, 6–3, 6–3 to win the men's singles tennis title at the 2016 Italian Open.
This was the first Italian Open in 11 years which was not won by either of Rafael Nadal or Djokovic.
The top eight seeds receive a bye into the second round.