The 2016 Internationaux de Tennis de Blois was a professional tennis tournament played on clay courts. It was the fourth edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Blois, France between 13 and 19 June 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Spain | Albert Montañés | 103 | 1 | |
 Argentina | Carlos Berlocq | 124 | 2 | |
| Thiemo de Bakker | 126 | 3 | ||
 Brazil | Thiago Monteiro | 138 | 4 | |
 Colombia | Alejandro González | 143 | 5 | |
 Belgium | Steve Darcis | 145 | 6 | |
 France | Mathias Bourgue | 153 | 7 | |
 China | Zhang Ze | 174 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
The following player received entry as a lucky loser:
See main article: 2016 Internationaux de Tennis de BLOIS – Singles.
Carlos Berlocq def. Steve Darcis, 6–2, 6–0See main article: 2016 Internationaux de Tennis de BLOIS – Doubles.
Gong Maoxin / 
Yasutaka Uchiyama, 6–3, 7–6(7–2)