The 2016 Hoff Open was a professional tennis tournament played on clay courts. It was the second edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Moscow, Russia between 6 and 12 June 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Kazakhstan | Mikhail Kukushkin | 83 | 1 | |
 Russian Federation | Karen Khachanov | 108 | 2 | |
 Moldova, Republic of | Radu Albot | 137 | 3 | |
| Russian Federation | Alexander Kudryavtsev | 156 | 4 | |
 Argentina | Facundo Argüello | 162 | 5 | |
 Brazil | André Ghem | 165 | 6 | |
| Kazakhstan | Aleksandr Nedovyesov | 174 | 7 | |
| Russian Federation | Andrey Rublev | 192 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry into the singles main draw with a protected ranking:
The following players received entry from the qualifying draw:
Emilio Gómez
Miki Janković Ronald Slobodchikov Alexander ZhurbinSee main article: 2016 Hoff Open – Singles.
Mikhail Kukushkin def. 
Steven Diez, 6–3, 6–3See main article: 2016 Hoff Open – Doubles.
Facundo Argüello / 
Roberto Maytín def. 
Aleksandre Metreveli / Dmitry Popko, 6–2, 7–5