The 2016 Guzzini Challenger was a professional tennis tournament played on hard courts. It was the fourteenth edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Recanati, Italy between 18 and 24 July 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Russian Federation | Evgeny Donskoy | 79 | 1 | |
 Ukraine | Illya Marchenko | 84 | 2 | |
 Hungary | Márton Fucsovics | 165 | 3 | |
 Colombia | Eduardo Struvay | 168 | 4 | |
 Belgium | Ruben Bemelmans | 177 | 5 | |
 Italy | Luca Vanni | 178 | 6 | |
| Russian Federation | Andrey Rublev | 193 | 7 | |
 Bosnia and Herzegovina | Aldin Šetkić | 207 | 8 |
The following players received wildcards into the singles main draw:
Riccardo Bonadio Andrea Vavassori Andrey Rublev Antonio MassaraThe following players entered the singles main draw with a protected ranking:
The following player entered the singles main draw as a special exempt:
The following players received entry from the qualifying draw:
The following player entered as a lucky losers:
| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
| Kevin Krawietz |  France | Albano Olivetti | 411 | 1 | ||
| Belgium | Ruben Bemelmans |  Spain | Adrián Menéndez Maceiras | 453 | 2 | |
| Ivan Sabanov | Matej Sabanov | 454 | 3 | |||
| Russian Federation | Alexandr Igoshin | Russian Federation | Yan Sabanin | 608 | 4 |
The following pairs received entry as wildcards:
Edoardo Lamberti / Panide Mangiaterra
Sam Barnett / Jesse WittenSee main article: 2016 Guzzini Challenger – Singles.
Illya Marchenko def. 
Ilya Ivashka, 6–4, 6–4See main article: 2016 Guzzini Challenger – Doubles.
Albano Olivetti def. Ruben Bemelmans / Adrián Menéndez Maceiras, 6–3, 7–6(7–4)