| Date: | 18 – 24 July |
| Edition: | 72nd |
| Category: | World Tour 250 series |
| Surface: | Clay / outdoor |
| Location: | Kitzbühel, Austria |
| Venue: | Tennis stadium Kitzbühel |
| Champs: |  Paolo Lorenzi |
| Champd: | Wesley Koolhof / Matwé Middelkoop |
The 2016 Generali Open Kitzbühel was a men's tennis tournament played on outdoor clay courts. It was the 72nd edition of the Austrian Open Kitzbühel, and part of the World Tour 250 series of the 2016 ATP World Tour. It took place at the Tennis stadium Kitzbühel in Kitzbühel, Austria, from July 18 through July 24.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Austria | Dominic Thiem | 9 | 1 | |
| Philipp Kohlschreiber | 22 | 2 | ||
 Spain | Marcel Granollers | 45 | 3 | |
| Italy | Paolo Lorenzi | 48 | 4 | |
 Czech Republic | Lukáš Rosol | 78 | 5 | |
 Serbia | Dušan Lajović | 81 | 6 | |
| Spain | Íñigo Cervantes | 82 | 7 | |
| Jan-Lennard Struff | 86 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
Diego Schwartzman →replaced by Michael Berrer| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
| Wesley Koolhof | Matwé Middelkoop | 104 | 1 | |||
| Argentina | Guillermo Durán | Argentina | Máximo González | 106 | 2 | |
 Israel | Jonathan Erlich |  Mexico | Santiago González | 116 | 3 | |
 Belarus | Aliaksandr Bury |  Slovakia | Igor Zelenay | 150 | 4 |
The following pairs received wildcards into the doubles main draw:
The following pair received entry as alternates:
See main article: 2016 Generali Open Kitzbühel – Singles.
Paolo Lorenzi defeated 
Nikoloz Basilashvili, 6–3, 6–4See main article: 2016 Generali Open Kitzbühel – Doubles.
Dennis Novak / Dominic Thiem, 2–6, 6–3, [11–9]
