| Date: | 2–7 May |
| Edition: | 8th |
| Draw: | 32S / 15D |
| Surface: | Clay |
| Prize Money: | €42,500+H |
| Location: | Rome, Italy |
| Champs: |  Kyle Edmund |
| Champd: |  Bai Yan / Li Zhe |
| Before Name: | Garden Open |
| After Name: | Garden Open |
The 2016 Garden Open was a professional tennis tournament played on clay courts. It was the eighth edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Rome, Italy between 2 and 7 May 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Czech Republic | Jiří Veselý | 63 | 1 | |
| United Kingdom | Kyle Edmund | 89 | 2 | |
 Argentina | Horacio Zeballos | 91 | 3 | |
| Czech Republic | Adam Pavlásek | 109 | 4 | |
 Serbia | Filip Krajinović | 110 | 5 | |
 Australia | Jordan Thompson | 118 | 6 | |
 France | Kenny de Schepper | 148 | 7 | |
| France | Vincent Millot | 149 | 8 |
The following players received wildcards into the singles main draw:
Federico Bonacia Flavio Cipolla Andrea Pellegrino Gianluca NasoThe following players received entry into the singles main draw: as a special exempt:
The following players received entry into the main draw with a protected ranking:
The following players received entry from the qualifying draw:
The following players entered the main draw as lucky losers:
See main article: 2016 Garden Open – Singles.
Kyle Edmund def. Filip Krajinović, 7–6(7–2), 6–0See main article: 2016 Garden Open – Doubles.
Bai Yan / Li Zhe def. Sander Arends / 
Tristan-Samuel Weissborn, 6–3, 3–6, [11–9]
