| Champ: | Rebeka Masarova |
| Runner: |  Amanda Anisimova |
| Score: | 7–5, 7–5 |
| Before Name: | French Open – Girls' singles |
| After Name: | French Open – Girls' singles |
See main article: 2016 French Open.
Rebeka Masarova won the title, defeating Amanda Anisimova in the final, 7–5, 7–5.
Paula Badosa was the defending champion, but was no longer eligible to participate in junior events.