| Date: | 13–19 June |
| Edition: | 17th (men) 6th (women) |
| Surface: | Hard |
| Prize Money: | $50,000 (men) $25,000 (women) |
| Location: | Fergana, Uzbekistan |
| Champms: |  Radu Albot |
| Champws: |  Polina Monova |
| Champmd: |  Yannick Jankovits / Luca Margaroli |
| Champwd: | Polina Monova / Yana Sizikova |
The 2016 Fergana Challenger was a professional tennis tournament played on hard courts. It was the 17th edition of the tournament for men which was part of the 2016 ATP Challenger Tour, offering a total of $50,000 in prize money, and the sixth edition of the event for women on the 2016 ITF Women's Circuit, offering a total of $25,000 in prize money. It took place in Fergana, Uzbekistan, on 13–19 June 2016.[1]
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Russian Federation | Konstantin Kravchuk | 109 | 1 | |
| Moldova, Republic of | Radu Albot | 113 | 2 | |
| Russian Federation | Alexander Kudryavtsev | 160 | 3 | |
 Kazakhstan | Dmitry Popko | 211 | 4 | |
 Korea, Republic of | Lee Duck-hee | 225 | 5 | |
 Ukraine | Denys Molchanov | 229 | 6 | |
 Serbia | Nikola Milojević | 240 | 7 | |
 Belarus | Ilya Ivashka | 247 | 8 |
The following players received wildcards into the singles main draw:
Sanjar Fayziev Jurabek Karimov Shonigmatjon Shofayziyev Khumoun SultanovThe following players received entry from the qualifying draw:
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Uzbekistan | Nigina Abduraimova | 174 | 1 | |
| Uzbekistan | Sabina Sharipova | 207 | 2 | |
| Russian Federation | Ksenia Lykina | 222 | 3 | |
| Ukraine | Valeriya Strakhova | 239 | 4 | |
| Russian Federation | Natela Dzalamidze | 269 | 5 | |
 India | Ankita Raina | 306 | 6 | |
| Russian Federation | Yana Sizikova | 338 | 7 | |
| Russian Federation | Veronika Kudermetova | 351 | 8 |
The following players received wildcards into the singles main draw:
Gozal Ainitdinova Shakhnoza Khatamova Sarvinoz Saidhujaeva Guzal YusupovaThe following players received entry from the qualifying draw:
Yuliya Kalabina Veronika Kapshay Varvara Kuznetsova Polina Merenkova Polina Monova
Alexandra Riley Jamilya Sadykzhanova Komola UmarovaThe following player received entry by a junior exempt:
See main article: 2016 Fergana Challenger – Men's singles.
Radu Albot def. Konstantin Kravchuk, 6–4, 6–2 Polina Monova def. Sabina Sharipova, 6–3, 0–6, 6–4See main article: 2016 Fergana Challenger – Men's doubles.
Yannick Jankovits / Luca Margaroli def. 
Toshihide Matsui / Vishnu Vardhan, 6–4, 7–6(7–4) Polina Monova / Yana Sizikova def. Prerna Bhambri / Ankita Raina, 7–6(7–0), 6–2
