| Match | Date | Team | Score | Team | Venue | Attendance |
|---|
| 1 | 2016.03.13 | FC Ryukyu | 1-0 | Fujieda MYFC | Okinawa Athletic Park Stadium | 1,311 |
| 2 | 2016.03.20 | FC Tokyo U-23 | 2-3 | FC Ryukyu | Ajinomoto Field Nishigaoka | 3,584 |
| 3 | 2016.04.02 | Cerezo Osaka U-23 | 2-1 | FC Ryukyu | Kincho Stadium | 1,408 |
| 4 | 2016.04.10 | FC Ryukyu | 1-0 | Oita Trinita | Okinawa Athletic Park Stadium | 1,424 |
| 5 | 2016.04.17 | Grulla Morioka | 2-3 | FC Ryukyu | Iwagin Stadium | 486 |
| 6 | 2016.04.24 | FC Ryukyu | 2-2 | Kataller Toyama | Okinawa Athletic Park Stadium | 948 |
| 7 | 2016.05.01 | FC Ryukyu | 2-2 | SC Sagamihara | Okinawa Athletic Park Stadium | 1,382 |
| 8 | 2016.05.08 | Gainare Tottori | 1-0 | FC Ryukyu | Tottori Bank Bird Stadium | 1,385 |
| 9 | 2016.05.15 | FC Ryukyu | 1-0 | Gamba Osaka U-23 | Okinawa Athletic Park Stadium | 1,251 |
| 10 | 2016.05.22 | Fukushima United FC | 1-1 | FC Ryukyu | Toho Stadium | 1,077 |
| 11 | 2016.05.29 | FC Ryukyu | 1-1 | Blaublitz Akita | Okinawa Athletic Park Stadium | 1,347 |
| 12 | 2016.06.12 | AC Nagano Parceiro | 2-0 | FC Ryukyu | Minami Nagano Sports Park Stadium | 3,986 |
| 13 | 2016.06.19 | FC Ryukyu | 0-1 | YSCC Yokohama | Okinawa Athletic Park Stadium | 1,554 |
| 14 | 2016.06.26 | Tochigi SC | 1-0 | FC Ryukyu | Tochigi Green Stadium | 4,824 |
| 15 | 2016.07.03 | FC Ryukyu | 3-1 | Kagoshima United FC | Okinawa Athletic Park Stadium | 1,357 |
| 16 | 2016.07.10 | Oita Trinita | 2-2 | FC Ryukyu | Oita Bank Dome | 7,051 |
| 17 | 2016.07.16 | FC Ryukyu | 1-5 | FC Tokyo U-23 | Okinawa Athletic Park Stadium | 1,146 |
| 18 | 2016.07.24 | Fujieda MYFC | 4-2 | FC Ryukyu | Fujieda Soccer Stadium | 1,508 |
| 19 | 2016.07.31 | FC Ryukyu | 0-3 | Cerezo Osaka U-23 | Okinawa Athletic Park Stadium | 4,565 |
| 20 | 2016.08.07 | Gamba Osaka U-23 | 1-4 | FC Ryukyu | Expo '70 Commemorative Stadium | 1,066 |
| 21 | 2016.09.11 | FC Ryukyu | 2-3 | Fukushima United FC | Okinawa Athletic Park Stadium | 1,115 |
| 22 | 2016.09.19 | FC Ryukyu | 4-2 | Grulla Morioka | Okinawa Athletic Park Stadium | 1,150 |
| 23 | 2016.09.25 | SC Sagamihara | 1-3 | FC Ryukyu | Sagamihara Gion Stadium | 2,536 |
| 24 | 2016.10.02 | FC Ryukyu | 1-1 | Tochigi SC | Okinawa Athletic Park Stadium | 1,473 |
| 25 | 2016.10.16 | YSCC Yokohama | 0-2 | FC Ryukyu | NHK Spring Mitsuzawa Football Stadium | 824 |
| 26 | 2016.10.23 | FC Ryukyu | 2-1 | AC Nagano Parceiro | Okinawa Athletic Park Stadium | 1,354 |
| 27 | 2016.10.30 | Kagoshima United FC | 1-2 | FC Ryukyu | Kagoshima Kamoike Stadium | 4,919 |
| 28 | 2016.11.06 | Kataller Toyama | 1-1 | FC Ryukyu | Toyama Stadium | 2,691 |
| 29 | 2016.11.13 | FC Ryukyu | 1-1 | Gainare Tottori | Okinawa Athletic Park Stadium | 2,041 |
| 30 | 2016.11.20 | Blaublitz Akita | 2-0 | FC Ryukyu | Akigin Stadium | 2,198 | |
Kim Jong-song