See main article: 2016 Engie Open de Biarritz.
The 2016 Engie Open de Biarritz – Singles was the singles event of the Open de Biarritz, a professional women's tennis tournament played on outdoor clay courts in Biarritz, France.
Laura Siegemund was the defending champion, but chose not to participate.
Rebecca Šramková won the title, defeating Martina Trevisan in the final, 6–3, 4–6, 6–1.