| Date: | 12–18 September |
| Edition: | 14th |
| Category: | ITF Women's Circuit |
| Location: | Biarritz, France |
| Prize Money: | $100,000 |
| Champs: |  Rebecca Šramková |
| Champd: |  Irina Khromacheva /  Maryna Zanevska |
The 2016 Engie Open de Biarritz Pays Basque was a professional tennis tournament played on outdoor clay courts. It was the 14th edition of the tournament and part of the 2016 ITF Women's Circuit, offering a total of $100,000 in prize money. It took place in Biarritz, France, on 12–18 September 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 France | Pauline Parmentier | 63 | 1 | |
 Romania | Sorana Cîrstea | 86 | 2 | |
| Carina Witthöft | 102 | 3 | ||
| Russian Federation | Irina Khromacheva | 118 | 4 | |
 Spain | Sílvia Soler Espinosa | 129 | 5 | |
 Brazil | Teliana Pereira | 136 | 6 | |
| Cindy Burger | 147 | 7 | ||
| Slovakia | Rebecca Šramková | 157 | 8 |
The following player received a wildcard into the singles main draw:
The following players received entry from the qualifying draw:
The following player received entry by a protected ranking:
See main article: 2016 Engie Open de Biarritz – Singles.
Rebecca Šramková def. 
Martina Trevisan, 6–3, 4–6, 6–1See main article: 2016 Engie Open de Biarritz – Doubles.
Irina Khromacheva / Maryna Zanevska def. 
Cornelia Lister / 
Nina Stojanović, 4–6, 7–5, [10–8]