| Date: | 9–15 May |
| Edition: | 8th |
| Category: | ITF Women's Circuit |
| Location: | Trnava, Slovakia |
| Prize Money: | $100,000 |
| Champs: |  Kateřina Siniaková |
| Champd: |  Anna Kalinskaya /  Tereza Mihalíková |
The 2016 Empire Slovak Open was a professional tennis tournament played on outdoor clay courts. It was the eighth edition of the tournament and part of the 2016 ITF Women's Circuit, offering a total of $100,000 in prize money. It took place in Trnava, Slovakia, on 9–15 May 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Slovakia | Magdaléna Rybáriková | 72 | 1 | |
 Turkey | Çağla Büyükakçay | 83 | 2 | |
| Anastasija Sevastova | 86 | 3 | ||
 Kazakhstan | Zarina Diyas | 94 | 4 | |
| Czech Republic | Kristýna Plíšková | 100 | 5 | |
 Poland | Magda Linette | 102 | 6 | |
 Romania | Andreea Mitu | 110 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
The following players received entry by lucky loser spots:
The following player received entry by a protected ranking:
See main article: 2016 Empire Slovak Open – Singles.
Kateřina Siniaková def. Anastasija Sevastova, 7–6(7–4), 5–7, 6–0See main article: 2016 Empire Slovak Open – Doubles.
Anna Kalinskaya / Tereza Mihalíková def. Evgeniya Rodina / Anastasija Sevastova, 6–1, 7–6(7–4)