| Champ: |  Víctor Estrella Burgos |
| Runner: |  Thomaz Bellucci |
| Score: | 4–6, 7–6(7–5), 6–2 |
| Draw: | 28 |
| Seeds: | 8 |
| Type: | singles doubles |
| Before Name: | Ecuador Open Quito – Singles |
| After Name: | Ecuador Open Quito – Singles |
See main article: 2016 Ecuador Open Quito. Víctor Estrella Burgos was the defending champion and successfully defended his title, defeating Thomaz Bellucci in the final, 4–6, 7–6(7–5), 6–2.
The top four seeds receive a bye into the second round.