| Date: | 21–27 November |
| Edition: | 3rd |
| Draw: | 32S / 16D |
| Surface: | Hard / Indoors |
| Prize Money: | $75,000 |
| Location: | Columbus, United States |
| Champs: |  Stefan Kozlov |
| Champd: |  David O'Hare /  Joe Salisbury |
| Before Name: | Columbus Challenger 1 |
| Before Year: | 2016 |
| Main Name: | Columbus Challenger |
| After Name: | Columbus Challenger |
The 2016 Columbus Challenger 2 was a professional tennis tournament played on indoor hard courts. It was the third edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Columbus, United States between 21 and 27 November 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| United States | Taylor Fritz | 70 | 1 | |
| United States | ||||
| Henri Laaksonen | 141 | 3 | ||
| United States | Stefan Kozlov | 141 | 4 | |
| United States | Mitchell Krueger | 211 | 5 | |
| United States | Tennys Sandgren | 232 | 6 | |
| United States | Austin Krajicek | 235 | 7 | |
| Ireland | Sam Barry | 284 | 8 |
The following players received entry into the singles main draw as wildcards:
Hugo Di Feo Martin Joyce
Herkko Pöllänen John McNallyThe following players received entry as alternates:
The following players received entry from the qualifying draw:
JC Aragone Hunter Callahan Aron Hiltzik Strong KirchheimerThe following player received entry as a lucky loser:
See main article: 2016 Columbus Challenger 2 – Singles.
Stefan Kozlov def. Tennys Sandgren 6–1, 2–6, 6–2.See main article: 2016 Columbus Challenger 2 – Doubles.
David O'Hare / Joe Salisbury def. Luke Bambridge / Cameron Norrie, 6–3, 6–4.