| Date: | 19–25 September |
| Edition: | 2nd |
| Draw: | 32S / 16D |
| Surface: | Hard / Indoors |
| Prize Money: | $50,000 |
| Location: | Columbus, United States |
| Champs: | Mikael Torpegaard |
| Champd: | Miķelis Lībietis /  Dennis Novikov |
| After Name: | Columbus Challenger 2 |
| After Year: | 2016 |
The 2016 Columbus Challenger 1 was a professional tennis tournament played on indoor hard courts. It was the second edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Columbus, United States between 19 and 25 September 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Benjamin Becker | 99 | 1 | ||
| United States | Dennis Novikov | 126 | 2 | |
| United States | Austin Krajicek | 151 | 3 | |
 Canada | Peter Polansky | 159 | 4 | |
| United States | Ernesto Escobedo | 175 | 5 | |
| Marcelo Arévalo | 178 | 6 | ||
| Blaž Rola | 189 | 7 | ||
 Brazil | Guilherme Clezar | 192 | 8 |
The following players received entry into the singles main draw as wildcards:
J. J. Wolf John McNally Martin JoyceThe following player received entry into the singles main draw as an alternate:
The following players received entry from the qualifying draw:
Jared Hiltzik
Roberto Quiroz Gonzales AustinSee main article: 2016 Columbus Challenger 1 – Singles.
See main article: 2016 Columbus Challenger 1 – Doubles.
Dennis Novikov def. Philip Bester / Peter Polansky, 7–5, 7–6(7–4).