| Date: | 26 September – 1 October |
| Edition: | 13th |
| Category: | ATP Challenger Tour |
| Location: | Medellín, Colombia |
| Surface: | Clay (Outdoor) |
| Prize Money: | $50,000+H |
| Champms: |  Facundo Bagnis |
| Champmd: |  Alejandro Falla / Eduardo Struvay |
| Before Name: | Seguros Bolívar Open Medellín |
The 2016 Claro Open Medellín was a professional tennis tournament played on outdoor clay courts. It is the 13th edition of the tournament. It was part of the 2016 ATP Challenger Tour. It took place in Medellín, Colombia, between 26 September and 1 October 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Dominican Republic | Víctor Estrella Burgos | 76 | 1 | |
| Argentina | Facundo Bagnis | 86 | 2 | |
 Brazil | Rogério Dutra Silva | 107 | 3 | |
| Argentina | Renzo Olivo | 120 | 4 | |
| Brazil | João Souza | 123 | 5 | |
| Colombia | Santiago Giraldo | 129 | 6 | |
| Argentina | Nicolás Kicker | 136 | 7 | |
| Colombia | Alejandro González | 168 | 8 |
The following players received wild cards into the singles main draw:
Gregorio Cordonnier Juan Sebastián Gómez Daniel Elahi Galán Sergio Luis Hernández RamírezThe following players received entry as alternates:
The following players received entry from the qualifying draw:
The following player received entry as a lucky loser:
See main article: 2016 Claro Open Medellín – Men's singles.
Facundo Bagnis def. Caio Zampieri 6–7(3–7), 7–5, 6–2.See main article: 2016 Claro Open Medellín – Men's doubles.
Alejandro Falla / Eduardo Struvay def. André Ghem / 
Juan Lizariturry 6–3, 6–2.