| Date: | 6–11 September |
| Edition: | 6th |
| Before Name: | Seguros Bolívar Open Barranquilla |
| Draw: | 32S / 16D |
| Surface: | Clay |
| Prize Money: | $50,000+H |
| Location: | Barranquilla, Colombia |
| Champs: |  Diego Schwartzman |
| Champd: |  Alejandro Falla / Eduardo Struvay |
The 2016 Claro Open Barranquilla was a professional tennis tournament played on clay courts. It was the fifth edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Barranquilla, Colombia between 6 and 11 September 2016.
| Country | Player | Rank | Seed | |
|---|---|---|---|---|
| Argentina | Diego Schwartzman | 69 | 1 | |
 Dominican Republic | Víctor Estrella Burgos | 83 | 2 | |
 Brazil | Rogério Dutra Silva | 108 | 3 | |
| Colombia | Santiago Giraldo | 132 | 4 | |
| Argentina | Máximo González | 146 | 5 | |
| Colombia | Alejandro González | 158 | 6 | |
| Darian King | 166 | 7 | ||
| Marcelo Arévalo | 176 | 8 |
The following players received wildcards into the singles main draw:
Santiago Giraldo Gregorio Cordonnier José Daniel Bendeck Víctor Estrella BurgosThe following player received entry into the singles main draw as an alternate:
The following players received entry from the qualifying draw:
See main article: 2016 Claro Open Barranquilla – Singles.
Diego Schwartzman def. Rogério Dutra Silva 6–4, 6–1.[1]See main article: 2016 Claro Open Barranquilla – Doubles.
Alejandro Falla / Eduardo Struvay def. 
Gonzalo Escobar / Roberto Quiroz 6–4,7–5.