The 2016 City of Onkaparinga ATP Challenger was a professional tennis tournament played on hard courts. It was the second edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Happy Valley, South Australia, Australia between 2–10 January 2016.
On 29 December 2015, Tennis South Australia Chief Executive Officer, Steven Baldas confirmed 15 players from inside the world's top 200 would compete, with Israel's Dudi Sela the highest ranked player in the field.[1]
| Country | Player | Rank | Seed | |
|---|---|---|---|---|
 Israel | Dudi Sela | 100 | 1 | |
| Bjorn Fratangelo | 128 | 2 | ||
 Colombia | Alejandro González | 141 | 3 | |
| 145 | 4 | |||
 Kazakhstan | 162 | 5 | ||
| 172 | 6 | |||
 Argentina | Renzo Olivo | 173 | 7 | |
| Taylor Fritz | 174 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
Taylor Fritz def. Dudi Sela 7–6(9–7), 6–2See main article: 2016 City of Onkaparinga ATP Challenger – Doubles.
Matteo Donati / Andrey Golubev def. 
Denys Molchanov / Aleksandr Nedovyesov 3–6, 7–6(7–5), [10–1]
