| Date: | 29 August – 4 September |
| Edition: | 11th |
| Surface: | Clay |
| Location: | Como, Italy |
| Champs: |  Kenny de Schepper |
| Champd: |  Roman Jebavý /  Andrej Martin |
The 2016 Città di Como Challenger was a professional tennis tournament played on clay courts. It was the eleventh edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Como, Italy between 29 August – 4 September 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Austria | Gerald Melzer | 91 | 1 | |
 Spain | Roberto Carballés Baena | 103 | 2 | |
 Japan | Taro Daniel | 104 | 3 | |
| Slovakia | Andrej Martin | 121 | 4 | |
 Argentina | Marco Trungelliti | 129 | 5 | |
| Argentina | Leonardo Mayer | 131 | 6 | |
| Spain | Albert Montañés | 135 | 7 | |
 Italy | Marco Cecchinato | 137 | 8 |
The following players received wildcards into the singles main draw:
The following player received entry as an alternate:
The following players received entry from the qualifying draw:
The following player received entry as a lucky loser:
See main article: 2016 Città di Como Challenger – Singles.
Kenny de Schepper def. Marco Cecchinato, 2–6, 7–6(7–0), 7–5.See main article: 2016 Città di Como Challenger – Doubles.
Roman Jebavý / Andrej Martin def. Nils Langer / Gerald Melzer, 3–6, 6–1, [10–5].
