The 2016 Città di Caltanissetta was a professional tennis tournament played on clay courts. It was the 18th edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Caltanissetta, Italy between 4 and 12 June 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Italy | Paolo Lorenzi | 54 | 1 | |
 Argentina | Facundo Bagnis | 99 | 2 | |
 Sweden | Elias Ymer | 118 | 3 | |
| Thiemo de Bakker | 120 | 4 | ||
 Colombia | Santiago Giraldo | 122 | 5 | |
| Colombia | Alejandro González | 142 | 6 | |
 Spain | Daniel Gimeno Traver | 160 | 7 | |
 Serbia | Peđa Krstin | 168 | 8 |
The following players received wildcards into the singles main draw:
Matteo Donati Omar Giacalone Paolo Lorenzi Gianluigi QuinziThe following player received entry into the singles main draw with a protected ranking:
The following players received entry from the qualifying draw:
Filip Horanský Antonio Massara
Sumit NagalSee main article: 2016 Città di Caltanissetta – Singles.
See main article: 2016 Città di Caltanissetta – Doubles.