| Date: | October 17–23 |
| Edition: | 11th |
| Category: | ITF Women's Circuit |
| Surface: | Hard – indoors |
| Location: | Saguenay, Quebec, Canada |
| Venue: | Club de tennis intérieur Saguenay |
| Champs: |  Catherine Bellis |
| Champd: |  Elena Bogdan / Mihaela Buzărnescu |
| Main Name: | Challenger de Saguenay |
The 2016 Challenger Banque Nationale de Saguenay was a professional tennis tournament played on indoor hard courts. It was the 11th edition of the tournament and part of the 2016 ITF Women's Circuit, offering a total of $50,000 in prize money. It took place in Saguenay, Quebec, Canada between October 17 and October 23, 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Catherine Bellis | 111 | 1 | ||
| Jennifer Brady | 112 | 2 | ||
| Grace Min | 132 | 3 | ||
| Sachia Vickery | 156 | 4 | ||
| Jessica Pegula | 165 | 5 | ||
 Serbia | Jovana Jakšić | 178 | 6 | |
 Canada | Françoise Abanda | 186 | 7 | |
 Japan | Mayo Hibi | 209 | 8 |
The following players received wildcards into the singles main draw:
Lauren Chypyha Gabriela Dabrowski Catherine Leduc Vanessa WongThe following players received entry from the qualifying draw:
The following player received entry by junior exempt:
See main article: 2016 Challenger Banque Nationale de Saguenay – Singles.
Catherine Bellis def. Bianca Andreescu, 6–4, 6–2See main article: 2016 Challenger Banque Nationale de Saguenay – Doubles.
Elena Bogdan / Mihaela Buzărnescu def. Bianca Andreescu / Charlotte Robillard-Millette, 6–4, 6–7(4–7), [10–6]