| Date: | 17–23 October |
| Edition: | 2nd |
| Surface: | Hard |
| Location: | Brest, France |
| Champs: |  Norbert Gombos |
| Champd: | Sander Arends /  Mateusz Kowalczyk |
The 2016 Brest Challenger was a professional tennis tournament played on hard courts. It was the second edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Brest, France between 17 and 23 October 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 France | Jérémy Chardy | 80 | 1 | |
| Slovakia | Lukáš Lacko | 116 | 2 | |
| Marco Chiudinelli | 120 | 3 | ||
 Italy | Alessandro Giannessi | 143 | 4 | |
 Canada | Steven Diez | 163 | 5 | |
| Italy | Luca Vanni | 168 | 6 | |
| France | ||||
 Serbia | Marko Tepavac | 181 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry using special exemptions:
The following players received entry from the qualifying draw:
The following players entered as a lucky losers:
See main article: 2016 Brest Challenger – Singles.
Norbert Gombos def. 
Yannik Reuter, 7–5, 6–2See main article: 2016 Brest Challenger – Doubles.
Mateusz Kowalczyk def. Marco Chiudinelli / Luca Vanni, 6–7(2–7), 6–3, [10–5]