| Champ: |  Mandy Minella |
| Runner: | Polona Hercog |
| Score: | 6–2, 6–3 |
| Type: | singles doubles |
| Before Year: | 2003 |
| Before Name: | Croatian Bol Ladies Open – Singles |
See main article: 2016 Bol Open.
This was the first edition of the tournament as a 125K event. Vera Zvonareva was the champion when it was last held as a Tier III event in 2003, but chose not to participate.
Mandy Minella won the title, defeating Polona Hercog in the final, 6–2, 6–3.