| Date: | 31 May – 5 June |
| Edition: | 11th |
| Category: | WTA 125K series |
| Surface: | Clay |
| Venue: | Bluesun Tennis Center Zlatni rat |
| Prize Money: | $125,000 |
| Location: | Bol, Croatia |
| Champs: |  Mandy Minella |
| Champd: | Xenia Knoll / Petra Martić |
| Before Year: | 2003 |
| Before Name: | Croatian Bol Ladies Open |
The 2016 Bol Open was a professional tennis tournament played on outdoor clay courts in Bol, Croatia. The tournament's first edition was held in late April 1991, and then again every year from 1996 to 2003. The tournament returned for 2016 as part of the WTA 125K series.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Slovakia | Anna Karolína Schmiedlová | 38 | 1 | |
| Varvara Lepchenko | 64 | 2 | ||
 China | Zhang Shuai | 69 | 3 | |
 Japan | Nao Hibino | 71 | 4 | |
| Ana Konjuh | 76 | 5 | ||
| Japan | Kurumi Nara | 91 | 6 | |
| Polona Hercog | 94 | 7 | ||
 Romania | Patricia Maria Țig | 99 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
The following players received entry as a lucky loser:
Denisa Allertová→replaced by Rebecca Peterson
Çağla Büyükakçay→replaced by Ysaline Bonaventure Sorana Cîrstea→replaced by Marina Erakovic
Mariana Duque Mariño→replaced by Julia Glushko
Kirsten Flipkens →replaced by Ivana Jorović
Yulia Putintseva →replaced by Paula Kania
Johanna Larsson →replaced by Petra Martić
Magda Linette →replaced by Sachia Vickery
Teliana Pereira →replaced by Kristína Kučová Rebecca Peterson→replaced by Ani Mijačika| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
| Romania | Raluca Olaru | Turkey | İpek Soylu | 170 | 1 | |
| Chan Chin-wei | Czech Republic | Renata Voráčová | 180 | 2 | ||
 Israel | Julia Glushko | Poland | Paula Kania | 214 | 3 | |
| Xenia Knoll | Petra Martić | 219 | 4 |
The following pairs received a wildcard into the doubles main draw:
See main article: 2016 Bol Open – Singles.
Mandy Minella def. Polona Hercog, 6–2, 6–3See main article: 2016 Bol Open – Doubles.
Raluca Olaru / İpek Soylu, 6–3, 6–2
