| Date: | 12–18 September |
| Edition: | 15th |
| Surface: | Clay |
| Champs: |  Adam Pavlásek |
| Champd: | Roman Jebavý / Jan Šátral |
The 2016 Banja Luka Challenger was a professional tennis tournament played on clay courts. It was the fifteenth edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Banja Luka, Bosnia and Herzegovina from 12 to 18 September 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Argentina | Horacio Zeballos | 71 | 1 | |
| Argentina | Carlos Berlocq | 76 | 2 | |
 United Kingdom | Aljaž Bedene | 77 | 3 | |
| Czech Republic | Adam Pavlásek | 112 | 4 | |
 Spain | Rubén Ramírez Hidalgo | 148 | 5 | |
 Hungary | Márton Fucsovics | 155 | 6 | |
 Serbia | Laslo Đere | 184 | 7 | |
| Serbia | Marko Tepavac | 185 | 8 |
The following players received wildcards into the singles main draw:
The following player received entry into the singles main draw with a protected ranking:
The following players received entry from the qualifying draw:
Maximilian Neuchrist Sebastian Ofner Lenny HampelSee main article: 2016 Banja Luka Challenger – Singles.
Adam Pavlásek def. Miljan Zekić, 3–6, 6–1, 6–4.See main article: 2016 Banja Luka Challenger – Doubles.
Roman Jebavý / Jan Šátral def. 
Andrea Arnaboldi / Maximilian Neuchrist, 7–6(7–2) 4–6, [10–7].