| Champ: | Jiske Griffioen |
| Runner: | Aniek van Koot |
| Score: | 6–3, 7–5 |
| Before Name: | Australian Open – Wheelchair women's singles |
| After Name: | Australian Open – Wheelchair women's singles |
See main article: 2016 Australian Open.
Defending champion Jiske Griffioen defeated Aniek van Koot in the final, 6–3, 7–5 to win the women's singles wheelchair tennis title at the 2016 Australian Open.