The 2016 Aspria Tennis Cup was a professional clay court tennis tournament that took place in Milan from 20–26 June. It was the 11th edition of the tournament and a part of the 2016 ATP Challenger Tour.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Brazil | Rogério Dutra Silva | 82 | 1 | |
| Gastão Elias | 91 | 2 | ||
 Spain | Roberto Carballés Baena | 111 | 3 | |
 Argentina | Carlos Berlocq | 119 | 4 | |
 Italy | Marco Cecchinato | 135 | 5 | |
 Colombia | Alejandro González | 147 | 6 | |
| Gonzalo Lama | 163 | 7 | ||
| Spain | Daniel Gimeno Traver | 173 | 8 |
The following players received wildcards into the singles main draw:
Matteo Donati Edoardo Eremin Gianluca Mager Gianluigi QuinziThe following player received entry into the singles main draw as a special exempt:
The following players received entry as alternates:
The following players received entry from the qualifying draw:
See main article: 2016 Aspria Tennis Cup – Singles.
Marco Cecchinato def. 
Laslo Đere, 6–2, 6–2See main article: 2016 Aspria Tennis Cup – Doubles.
Miguel Ángel Reyes-Varela / 
Max Schnur def. Alessandro Motti / Peng Hsien-yin, 1–6, 7–6(7–4), [10–5]
