The women's 400 metres hurdles event at the 2016 African Championships in Athletics was held on 25 and 26 June in Kings Park Stadium.[1]
| width=200 bgcolor=gold | Gold | width=200 bgcolor=silver | Silver | width=200 bgcolor=CC9966 | Bronze |
| Wenda Nel | Maureen Jelagat | Tameka Jameson |
Qualification: First 3 of each heat (Q) and the next 2 fastest (q) qualified for the final.
| Rank | Heat | Name | Nationality | Time | Notes | |
|---|---|---|---|---|---|---|
| 1 | 1 | 55.33 | Q | |||
| 2 | 2 | 57.63 | Q | |||
| 3 | 2 | 58.17 | Q | |||
| 4 | 2 | 58.50 | Q | |||
| 5 | 1 | 58.55 | Q | |||
| 6 | 2 | 58.59 | q | |||
| 7 | 1 | 59.44 | Q | |||
| 8 | 1 | 59.55 | q | |||
| 9 | 1 | 59.70 | ||||
| 10 | 2 | |||||
| 2 |
| Rank | Lane | Athlete | Nationality | Time | Notes | |
|---|---|---|---|---|---|---|
| 5 | 54.86 | |||||
| 4 | 56.12 | |||||
| 6 | 57.17 | |||||
| 4 | 2 | 57.85 | ||||
| 5 | 8 | 58.60 | ||||
| 6 | 7 | 59.08 | ||||
| 7 | 3 | 59.19 | ||||
| 8 | 1 | 59.78 |