| Champ: |  Dominika Cibulková |
| Runner: |  Karolína Plíšková |
| Score: | 7–5, 6–3 |
| Draw: | 48 |
| Seeds: | 16 |
| Type: | singles doubles |
| Before Name: | Aegon International |
| After Name: | Aegon International Eastbourne – Women's singles |
See main article: article and 2016 Aegon International Eastbourne. Belinda Bencic was the defending champion, but lost in the second round to Elena Vesnina.
Dominika Cibulková won the title, defeating Karolína Plíšková in the final, 7–5, 6–3.
All seeds received a bye into the second round.