| Date: | 13–19 June |
| Edition: | 2nd |
| Category: | ATP Challenger Tour ITF Women's Circuit |
| Location: | Ilkley, United Kingdom |
| Prize Money: | €42,500 (ATP) $50,000 (ITF) |
| Champms: | Lu Yen-hsun |
| Champws: |  Evgeniya Rodina |
| Champmd: | Wesley Koolhof / Matwé Middelkoop |
| Champwd: |  Yang Zhaoxuan / Zhang Kailin |
The 2016 Aegon Ilkley Trophy was a professional tennis tournament played on outdoor grass courts. It was the second edition of the tournament for both men and women. It was part of the 2016 ATP Challenger Tour and the 2016 ITF Women's Circuit, offering a total prize money of €42,500 for men and $50,000 for women. It took place in Ilkley, United Kingdom, on 13–19 June 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Australia | Jordan Thompson | 90 | 1 | |
| Lu Yen-hsun | 97 | 2 | ||
 United States | Bjorn Fratangelo | 99 | 3 | |
| Igor Sijsling | 118 | 4 | ||
| United States | Tim Smyczek | 123 | 5 | |
 Bosnia and Herzegovina | Mirza Bašić | 130 | 6 | |
| Marco Chiudinelli | 131 | 7 | ||
| United States | Austin Krajicek | 135 | 8 |
The following players received wildcards into the singles main draw:
The following player received entry as a special exemption into the singles main draw:
The following players received entry from the qualifying draw:
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| China | Zhang Kailin | 115 | 1 | |
| Russian Federation | Evgeniya Rodina | 120 | 2 | |
| Russian Federation | Irina Khromacheva | 121 | 3 | |
| China | Han Xinyun | 123 | 4 | |
| China | Duan Yingying | 131 | 5 | |
| Richèl Hogenkamp | 134 | 6 | ||
 Ukraine | Maryna Zanevska | 138 | 7 | |
 Serbia | Ivana Jorović | 145 | 8 |
The following player received a wildcard into the singles main draw:
The following players received entry from the qualifying draw:
The following player received entry by a lucky loser spot:
The following player received entry by a protected ranking:
The following player received entry by a special exemption:
See main article: 2016 Aegon Ilkley Trophy – Men's singles.
Vincent Millot, 7–6(7–4), 6–2 Evgeniya Rodina def. 
Rebecca Šramková, 6–4, 6–4
Marcelo Demoliner / 
Aisam-ul-Haq Qureshi, 7–6(7–5), 0–6, [10–8] Yang Zhaoxuan / Zhang Kailin def. 
An-Sophie Mestach / Storm Sanders, 6–3, 7–6(7–5)
