| Date: | 25–31 July |
| Edition: | 23rd (men) 12th (women) |
| Category: | ATP Challenger Tour ITF Women's Circuit |
| Location: | Prague, Czech Republic |
| Venue: | I. Czech Lawn Tennis Club |
| Prize Money: | €42,500 (men) $75,000 (women) |
| Champms: |  Santiago Giraldo |
| Champws: | Antonia Lottner |
| Champmd: |  Julian Knowle /  Igor Zelenay |
| Champwd: | Demi Schuurs /  Renata Voráčová |
The 2016 Advantage Cars Prague Open , also known as Advantage Cars Prague Open by Zenova for sponsorship reasons, was a professional tennis tournament played on outdoor clay courts. It was the 23rd edition, for men, and 12th edition, for women, of the tournament and part of the 2016 ATP Challenger Tour and the 2016 ITF Women's Circuit, offering totals of €42,500, for men, and $50,000, for women, in prize money. It took place in Prague, Czech Republic, on 25–31 July 2016.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Slovakia | Martin Kližan | 28 | 1 | |
 Moldova, Republic of | Radu Albot | 89 | 2 | |
 Georgia | Nikoloz Basilashvili | 123 | 3 | |
| Slovakia | Jozef Kovalík | 126 | 4 | |
 Spain | Daniel Gimeno-Traver | 131 | 5 | |
| Colombia | Santiago Giraldo | 150 | 6 | |
| Henri Laaksonen | 165 | 7 | ||
 Argentina | Facundo Argüello | 173 | 8 |
The following player received a wildcard into the singles main draw:
Martin Kližan Václav Šafránek David Šimůnek Lukáš VejvaraThe following players received entry from the qualifying draw:
Hubert Hurkacz
Vadym Ursu Matěj Vocel Igor Zelenay| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Mona Barthel | 79 | 1 | ||
| Czech Republic | Denisa Allertová | 83 | 2 | |
 Italy | Karin Knapp | 85 | 3 | |
| Carina Witthöft | 94 | 4 | ||
| Ukraine | Kateryna Kozlova | 96 | 5 | |
 Russian Federation | Evgeniya Rodina | 104 | 6 | |
| Russian Federation | Elizaveta Kulichkova | 115 | 7 | |
| Spain | Sílvia Soler Espinosa | 127 | 8 |
The following player received a wildcard into the singles main draw:
Simona Heinová Andrea Hlaváčková Karolína Muchová Katarzyna PiterThe following players received entry from the qualifying draw:
The following player received entry by a lucky loser spot:
See main article: 2016 Advantage Cars Prague Open – Men's singles.
Santiago Giraldo def. 
Uladzimir Ignatik, 6–4, 3–6, 7–6(7–2)[1]See main article: 2016 Advantage Cars Prague Open – Women's singles.
See main article: 2016 Advantage Cars Prague Open – Men's doubles.
Julian Knowle / Igor Zelenay def. Facundo Argüello / Julio Peralta, 6–4, 7–5[2]See main article: 2016 Advantage Cars Prague Open – Women's doubles.
Renata Voráčová def. Sílvia Soler Espinosa / Sara Sorribes Tormo, 7–5, 3–6, [10–4]