The 2016 ATP Shenzhen Open was a professional men's tennis tournament played on hard courts. It was the third edition of the tournament, and part of the ATP World Tour 250 series of the 2016 ATP World Tour. It took place at the Shenzhen Longgang Tennis Centre in Shenzhen, China from September 26 to October 2.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Czech Republic | Tomáš Berdych | 9 | 1 | |
 Belgium | David Goffin | 14 | 2 | |
 France | Richard Gasquet | 17 | 3 | |
 Australia | Bernard Tomic | 22 | 4 | |
| France | Benoît Paire | 38 | 6 | |
 Italy | Fabio Fognini | 43 | 7 | |
| Czech Republic | Jiří Veselý | 51 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry using a protected ranking:
The following players received entry from the qualifying draw:
The following player received entry as a lucky loser:
Alexandr Dolgopolov →replaced by 
Íñigo Cervantes
Daniel Evans →replaced by Lukáš Rosol
Taylor Fritz →replaced by 
Yoshihito Nishioka Thomas Fabbiano| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
| Treat Huey |  Belarus | Max Mirnyi | 41 | 1 | ||
 Austria | Oliver Marach | France | Fabrice Martin | 67 | 2 | |
| Mate Pavić |  New Zealand | Michael Venus | 75 | 3 | ||
| Australia | Chris Guccione |  Brazil | André Sá | 88 | 4 |
The following pairs received wildcards into the doubles main draw:
See main article: 2016 ATP Shenzhen Open – Singles.
Tomáš Berdych def. Richard Gasquet, 7–6(7–5), 6–7(2–7), 6–3[1]See main article: 2016 ATP Shenzhen Open – Doubles.
Fabio Fognini / 
Robert Lindstedt def. Mate Pavić / Michael Venus, 7–6(7–4), 6–3
