| Season: | 2016–17 |
| League Topscorer: | Jakub Plšek (18 goals) |
| Matches: | 240 |
| Total Goals: | 676 |
| Nextseason: | 2017–18 |
The 2016–17 Czech National Football League was the 24th season of the Czech Republic's second tier football league.
After one season played with 15 teams, the number of teams in the Czech National Football League increased back to 16 for the 2016–17 season.
| Club | Location | Stadium | Capacity | 2015-16 Position | |
|---|---|---|---|---|---|
| Baník Ostrava | Ostrava | 15,123 | 16th in 1. Liga | ||
| České Budějovice | České Budějovice | 6,681 | 12th | ||
| Frýdek-Místek | Frýdek-Místek | 12,000 | 10th | ||
| Opava | Opava | 7,758 | 11th | ||
| Pardubice | Pardubice | 2,500 | 6th | ||
| Prostějov | Prostějov | 3,500 | 1st in MSFL | ||
| Sigma Olomouc | Olomouc | 12,566 | 15th in 1. Liga | ||
| Sokolov | Sokolov | 5,000 | 4th | ||
| Táborsko | Sezimovo Ústí | 5,000 | 5th | ||
| Třinec | Třinec | 2,200 | 13th | ||
| Ústí nad Labem | Ústí nad Labem | 3,000 | 7th | ||
| Varnsdorf | Varnsdorf | 5,000 | 9th | ||
| Viktoria Žižkov | Prague | 5,600 | 3rd in ČFL | ||
| Vítkovice | Vítkovice | 15,123 | 2nd in MSFL | ||
| Vlašim | Vlašim | 6,000 | 8th | ||
| Znojmo | Znojmo | 5,000 | 3rd |