The 2014 Winston-Salem Open was a men's tennis tournament played on outdoor hard courts. It was the 46th edition of the Winston-Salem Open (as successor to previous tournaments in New Haven and Long Island), and was part of the ATP World Tour 250 Series of the 2014 ATP World Tour. It took place at the Wake Forest University in Winston-Salem, North Carolina, United States, from August 17 through August 23, 2014. It was the last event on the 2014 US Open Series before the 2014 US Open.
| Country | Player | Rank* | Seed | |
|---|---|---|---|---|
| 14 | 1 | |||
| 17 | 2 | |||
 Spain | 20 | 3 | ||
 Argentina | 26 | 4 | ||
| Spain | 32 | 5 | ||
| 37 | 6 | |||
 Czech Republic | 38 | 7 | ||
| Spain | Marcel Granollers | 39 | 8 | |
| 43 | 9 | |||
| Spain | 47 | 10 | ||
| 48 | 11 | |||
 France | 49 | 12 | ||
 Kazakhstan | 51 | 13 | ||
 Italy | 52 | 14 | ||
 Finland | 53 | 15 | ||
| Steve Johnson | 55 | 16 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
Alejandro Falla
Teymuraz Gabashvili Santiago Giraldo
Vasek Pospisil
Jack Sock Dmitry Tursunov Jiří Veselý John Isner (ankle injury)| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
| Spain | David Marrero |  India | Leander Paes | 22 | 1 | |
| India | Rohan Bopanna | Canada | Daniel Nestor | 26 | 2 | |
 United Kingdom | Jamie Murray |  Australia | John Peers | 59 | 3 | |
| United Kingdom |
The following pairs received wildcards into the doubles main draw:
The following pair used a protected ranking to gain entry into the doubles main draw:
The following pair received entry as alternates:
Dominic Inglot (abdominal injury) David Marrero (back injury)See main article: 2014 Winston-Salem Open – Singles. Lukáš Rosol def. 
Jerzy Janowicz, 3–6, 7–6(7–3), 7–5
See main article: 2014 Winston-Salem Open – Doubles. Juan Sebastián Cabal / Robert Farah def. Jamie Murray / John Peers, 6–3, 6–4
