The 2014 UniCredit Czech Open was a professional tennis tournament played on clay courts. It was the 21st edition of the tournament which was part of the 2014 ATP Challenger Tour. It took place in Prostějov, Czech Republic between 2 and 7 June 2014.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
 Czech Republic | Radek Štěpánek | 43 | 1 | |
| Robin Haase | 50 | 2 | ||
| Czech Republic | Lukáš Rosol | 52 | 3 | |
 Kazakhstan | Mikhail Kukushkin | 54 | 4 | |
| Bradley Klahn | 71 | 5 | ||
| Czech Republic | Jiří Veselý | 81 | 6 | |
| Julian Reister | 91 | 7 | ||
 India | Somdev Devvarman | 96 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry as alternates into the singles main draw:
The following players received entry from the qualifying draw:
Tristan Lamasine Daniel Knoflicek Julien Obry Michal Konečný| Country | Player | Country | Player | Rank1 | Seed | |
|---|---|---|---|---|---|---|
 Poland | Tomasz Bednarek | Czech Republic | Lukáš Dlouhý | 101 | 1 | |
| Andre Begemann | Czech Republic | Lukáš Rosol | 102 | 2 | ||
| Martin Emmrich | Christopher Kas | 111 | 3 | |||
| Czech Republic | František Čermák |  Russian Federation | Mikhail Elgin | 123 | 4 |
The following pairs received wildcards into the doubles main draw:
Zdeněk Kolář / David Poljak Michal Konečný / Jaroslav Pospíšil Adam Pavlásek / Jiří Veselý Jiří Veselý def. 
Norbert Gombos, 6–2, 6–2 Lukáš Rosol def. 
Peter Polansky / Adil Shamasdin, 6–1, 6–2