| Date: | 12–18 May |
| Edition: | 5th |
| Category: | ITF Women's Circuit |
| Location: | Prague, Czech Republic |
| Surface: | Clay |
| Venue: | TK Sparta Prague |
| Prize Money: | $100,000+H |
| Champs: |  Heather Watson |
| Champd: |  Lucie Hradecká / Michaëlla Krajicek |
| After Name: | J&T Banka Prague Open |
The 2014 Sparta Prague Open was a professional tennis tournament played on outdoor clay courts. It was the fifth edition of the tournament and part of the 2014 ITF Women's Circuit, offering a total of $100,000+H in prize money. It took place in Prague, Czech Republic, from 12 to 18 May 2014.
| Country | Player | Rank1 | Seed | |
|---|---|---|---|---|
| Czech Republic | Lucie Šafářová | 25 | 1 | |
| Czech Republic | Klára Koukalová | 31 | 2 | |
| Czech Republic | Barbora Záhlavová-Strýcová | 55 | 3 | |
 Belgium | Yanina Wickmayer | 58 | 4 | |
| Czech Republic | Karolína Plíšková | 65 | 5 | |
 Slovakia | Anna Karolína Schmiedlová | 68 | 6 | |
| Dinah Pfizenmaier | 82 | 7 | ||
 Japan | Misaki Doi | 91 | 8 |
The following players received wildcards into the singles main draw:
Simona Heinová Kateřina Siniaková Tereza Smitková Petra UberalováThe following players received entry from the qualifying draw:
The following player received entry by a special exempt:
See main article: 2014 Sparta Prague Open – Singles.
Heather Watson def. Anna Karolína Schmiedlová 7–6(7–5), 6–0See main article: 2014 Sparta Prague Open – Doubles.
Lucie Hradecká / Michaëlla Krajicek def. Andrea Hlaváčková / Lucie Šafářová 6–3, 6–2
