The 2014 Sarasota Open was a professional tennis tournament played on clay courts. It was the sixth edition of the tournament which was part of the 2014 ATP Challenger Tour. It took place in Sarasota, Florida, United States between April 14 and April 20, 2014.
| Country | Player | Rank | Seed | |
|---|---|---|---|---|
 Russian Federation | Alex Bogomolov Jr. | 85 | 1 | |
| Donald Young | 91 | 2 | ||
| Jack Sock | 94 | 3 | ||
| Michael Russell | 97 | 4 | ||
| Tim Smyczek | 103 | 5 | ||
| Alex Kuznetsov | 124 | 6 | ||
 Argentina | Facundo Bagnis | 132 | 7 | |
 Canada | Peter Polansky | 135 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
Gianni Mina 
Naoki Nakagawa| Country | Player | Country | Player | Rank | Seed | |
|---|---|---|---|---|---|---|
| Scott Lipsky |  New Zealand | Michael Venus | 110 | 1 | ||
| Marin Draganja |  Finland | Henri Kontinen | 122 | 2 | ||
 United Kingdom | Ken Skupski | United Kingdom | Neal Skupski | 132 | 3 | |
| Gero Kretschmer | Alexander Satschko | 167 | 4 |
The following pairs received wildcards into the doubles main draw:
Sekou Bangoura / Vahid Mirzadeh Siddhartha Chappidi / Joel Link Jarmere Jenkins / Mac StyslingerThe following pairs received entry from the qualifying draw:
See main article: 2014 Sarasota Open – Singles.
Nick Kyrgios def. 
Filip Krajinović, 7–6(12–10), 6–4See main article: 2014 Sarasota Open – Doubles.
Henri Kontinen def. 
Rubén Ramírez Hidalgo / Franko Škugor, 7–5, 5–7, [10–6]